Two DU145 clones, with low and high junB expression, were transfected with GFP-encoding plasmids and plated on chick chorioallantoic membrane (CAM) to investigate the potential for invasion. After transfection, the clones were seeded atop the CAM of 11-day-old chicks and cultured for 3 or 5 days. More numerous macroscopic allantoic vessels radiating in a wheel pattern were seen toward the low junB clone than toward the high junB clone. CAM tissues were fixed and stained with anti-GFP and the invading cell numbers and the depth of invasion were quantified from the CAM surface. For more details, please see Konishi and coworkers on page 4408 in this issue.

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